Anti-IRS4 Antibody Picoband® A04616-1 from BosterBio

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Anti-IRS4 Antibody Picoband®

Western blot analysis of IRS4 using anti-IRS4 antibody (A04616-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: rat brain whole cell lysates,
Lane 3: rat testis whole cell lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRS4 antigen affinity purified polyclonal antibody (Catalog # A04616-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRS4 at approximately 150 kDa. The expected band size for IRS4 is at 134 kDa.
Anti-IRS4 Antibody Picoband®

IHC analysis of IRS4 using anti-IRS4 antibody (A04616-1).
IRS4 was detected in a paraffin-embedded section of diffuse large B-cell lymphoma of human intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IRS4 Antibody (A04616-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Anti-IRS4 Antibody Picoband®

IHC analysis of IRS4 using anti-IRS4 antibody (A04616-1).
IRS4 was detected in a paraffin-embedded section of diffuse large B-cell lymphoma of human intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IRS4 Antibody (A04616-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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BosterBio for
Anti-IRS4 Antibody Picoband®

The Anti-IRS4 Antibody Picoband® from BosterBio is a Rabbit Polyclonal antibody to IRS4. This antibody recognizes Homo Sapiens, Human, Mouse, and Rat antigen. The Anti-IRS4 Antibody Picoband® has been validated for the following applications: EIA, Immunoassay, ELISA, Flow Cytometry, Immunohistochemistry, and Western Blot.

Description

Boster Bio Anti-IRS4 Antibody Picoband™ catalog # A04616-1. Tested in ELISA, IHC, WB, Flow Cytometry (Intracellular) applications. This antibody reacts with Human, Mouse, Rat

Anti-IRS4 Antibody Picoband®

Anti-IRS4 Antibody Picoband®

Anti-IRS4 Antibody Picoband®

Anti-IRS4 Antibody Picoband® from BosterBio

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Anti-IRS4 Antibody Picoband® from BosterBio

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